Ying Ge, Ph.D.

Dr. Ying GeDirector of Mass Spectrometry
Human Proteomics Program
School of Medicine and Public Health
University of Wisconsin - Madison

Education
B.S. 1997, Peking (Beijing) University, China
Ph.D. 2002, Cornell University

Contact Information
Email: yge@physiology.wisc.edu
(608) 263-9212 Phone
(608) 265-5512 Fax

Research Interests

Develop and apply cutting-edge mass spectrometric technology to biomedical research:

  1. Top-down disease proteomics - deciphering protein modifications for understanding and diagnosis of human diseases

  2. Characterization of complex post-translational modifications in large proteins (140 kDa) by high resolution tandem mass spectrometry with electron capture dissociation

  3. Discovery, characterization and validation of biomarkers associated with human disease

Selected Publications

  1. Ge, Y.; Rybakova, I.; Xu, Q.; Moss, R. L. Top-down high resolution mass spectrometry of cardiac myosin binding protein C revealed that truncation alters protein phosphorylation state, Proc. Natl. Acad. Sci. U. S. A., 2009, Epub ahead of print. (Ge, Y. Corresponding author) *This article is a PNAS Direct Submission (Track II).

  2. Ayaz-Guner, S.; Zhang, J.; Li, L.; Walker, J. W.; Ge, Y. In Vivo Phosphorylation Site Mapping in Mouse Cardiac Troponin I by High Resolution Top-Down Electron Capture Dissociation Mass Spectrometry:  Ser22/23 Are the Only Sites Basally Phosphorylated, Biochemistry, Epub ahead of print. (Ge, Y. Corresponding author)

  3. Nelson, C. A.; Szczech, J. R.; Xu, Q.; Jin, S.; Ge, Y. Mesoporous Metal Oxide Nanomaterials Effectively Enrich Phosphopeptides for Mass Spectrometry-based Phosphoproteomics, Chem. Commun. In Revision. (Ge, Y. Corresponding author)

  4. Kuhn, P.; Xu, Q.; Cline, E.; Zhang, D.; Ge, Y.; Xu, W. Delineating Anopheles gambiae coactivator  associated arginine methyltransferase 1 (AgCARM1) by top-down high resolution tandem mass spectrometry, Protein Sci. 2009, 18, 1272-1280. (Ge, Y. Corresponding author)

  5. Liu, L.; Hou, J.; Du, J.; Chumanov, R. S. ; Xu, Q.; Ge, Y.; Johnson, J.A.; Murphy, R.M. Differential modification of Cys10 alters transthyretin’s effect on beta-amyloid aggregation and toxicity, Protein Eng. Des. Sel. 2009, 1–10.
  6. Ma, M.; Chen, R.; Ge, Y.; He, H; Marshall, A. G.; Li, L. Combining bottom-up and top-down mass spectrometric strategies for de novo sequencing of the Crustacean hyperglycemic hormone (CHH) from Cancer borealis, Anal. Chem. 2009, 81, 240-247.

  7. Sancho Solis, R.; Ge, Y.; Walker, J. W. Single amino acid sequence polymorphisms in rat cardiac troponin revealed by top-down tandem mass spectrometry, J. Muscle Res. Cell Motil. 2008, 29, 203-212.

  8. Zabrouskov, V.; Ge, Y.; Schwartz, J.; Walker, J. W. Unraveling molecular complexity of phosphorylated human cardiac troponin I by top down electron capture dissociation/electron transfer dissociation mass spectrometry, Mol. Cell. Proteomics 2008, 7, 1838-1849. (Ge, Y. Corresponding author)

  9. Colabroy, K. L.; Zhai, H. L.; Li, T. F.; Ge, Y.; Zhang, Y.; Liu, A. M.; Ealick, S. E.; McLafferty, F. W.;   Begley, T. P., The mechanism of inactivation of 3-hydroxyanthranilate-3,4-dioxygenase by 4-chloro-3-hydroxyanthranilate. Biochemistry 2005, 44, 7623-7631.

  10. Xiang, J.; Ipek, M.; Suri, V.; Massefski, W.; Pan, N.; Ge, Y.; Tam, M.; Xing, Y.; Tobin, J.; Xu, X.; Tam, S. Synthesis and biological evaluation of sulfonamidooxazoles and b-keto sulfones: selective inhibitors of 11b–hydroxyster oid dehydrogenase type I, Bioorg. Med. Chem. Lett. 2005, 15, 2865-2869.

  11. Ge, Y.; Lawhorn, B. G.; ElNaggar, M.; Sze, S. K.; Begley, T. P.; McLafferty, F. W.  Protein Oxidation in Viral Prolyl-4-hydroxylase Characterized by Top Down High Resolution Tandem Mass Spectrometry, Protein Sci. 2003, 12, 2320-2326.

  12. Ge, Y.; ElNaggar, M.; Sze, N.; Han Bin Oh, Begley, T. P.; McLafferty, F. W.; Boshoff, H.; Barry, C. B. Top Down Characterization of Secreted Proteins from Mycobacterium Tuberculosis by Electron Capture Dissociation Mass Spectrometry J. Am. Soc. Mass Spectrom. 2003 14, 253-261.

  13. Sze, S. K.; Ge, Y.; Oh, H.; McLafferty, F. W.; Plasma Electron Capture Characterization of Large Dissociation for the Proteins by Top Down Mass Spectrometry Anal. Chem. 2003, 75, 1599-1603.

  14. Oh, H.; Bruker, K.; Sze, S. K.; Ge, Y.; McLafferty, F. W.; Secondary and Tertiary Structure of Gaseous Ions Characterized by Electron Capture Dissociation Mass Spectrometry and Photofragment Spectroscopy, Proc. Natl. Acad. Sci. U. S. A. 2002, 99, 15863-15868.

  15. Sze, S. K.; Ge, Y.; Oh, H.; McLafferty, F. W.; Top Down Mass Spectrometry of a 29 kDa protein for Characterization of Any Posttranslational Modification to Within One Residue, Proc. Natl. Acad. Sci. U. S. A. 2002, 99, 1774-1779.

  16. Ge, Y.; Lawhorn, B. G.; ElNaggar, M.; Strauss, E.; Park, J-H.; Begley, T. P.; McLafferty, F. W.  “Top Down” Characterization of Larger Proteins (45 kDa) by Electron Capture Dissociation Mass Spectrometry, J. Am. Chem. Soc. 2002, 124, 672-678.

  17. Xi, J.; Ge, Y.; Kinsland, C. L.; McLafferty, F. W.; Begley, T. P.  Biosynthesis of the Thiazole Moiety of Thiamin in Echeriachia Coli: Identification of a Novel Acyldisulfide Linked Protein-protein Conjugate that Is Functionally Analogous to the Ubiquitin/E1 Complex, Proc. Natl. Acad. Sci. U. S. A. 2001, 98, 8513-8518.

  18. Ge, Y.; Horn, D. M.; McLafferty, F. W.  Blackbody Infrared Radiative Dissociation of Larger (42 kDa) Multiply Charged Proteins, Int. J. Mass Spectrom. 2001, 210/211, 203-214.

  19. McLafferty, F. W.; Horn, D. M.; Ge, Y.; Cerda, B.; Fridriksson, E.; Breuker, K.  New Frontiers in Molecular Mass Spectrometry, Adv. Mass Spectrom. 2001, 67, 167-173.

  20. McLafferty, F. W.; Horn, D. M.; Breuker, K.; Ge, Y;  Cerda, B., A. Electron Capture Dissociation of Gaseous Multiply Charged Ions by FTICR,  J. Am. Soc. Mass Spectrom. 2001, 12, 245-249.

  21. Strauss, E.; Kinsland, C.; Ge, Y.; McLafferty, F. W.; Begley, T. P.  The Identification and Characterization of Phosphopantothenoylcysteine Synthetase from E.coli Completes the Identification of Coenzyme A Biosynthetic Enzymes in Bacteria, J. Biol. Chem. 2001, 276, 13513.

  22. Horn, D. M.; Ge, Y.; McLafferty, F. W. Activated Ion Electron Capture Dissociation for Mass Spectral Sequencing for Larger (42 kDa) Proteins, Anal. Chem. 2000, 72, 4778-4784.